AccPbFRET: an ImageJ plugin for semi-automatic, fully corrected analysis of acceptor photobleaching FRET images.

ABSTRACT:

Background

The acceptor photobleaching fluorescence resonance energy transfer (FRET) method is widely used for monitoring molecular interactions in cells. This method of FRET, while among those with the simplest mathematics, is robust, self-controlled and independent of fluorophore amounts and ratios.

Results

AccPbFRET is a user-friendly, efficient ImageJ plugin which allows fully corrected, pixel-wise calculation and detailed, ROI (region of interest)-based analysis of FRET efficiencies in microscopic images. Furthermore, automatic registration and semi-automatic analysis of large image sets is provided, which are not available in any existing FRET evaluation software.

Conclusion

Despite of the widespread applicability of the acceptor photobleaching FRET technique, t

SUBMITTER: Roszik J 

PROVIDER: S-EPMC2571114 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature