Unassigned MURF1 of kinetoplastids codes for NADH dehydrogenase subunit 2.

ABSTRACT:

Background

In a previous study, we conducted a large-scale similarity-free function prediction of mitochondrion-encoded hypothetical proteins, by which the hypothetical gene murf1 (maxicircle unidentified reading frame 1) was assigned as nad2, encoding subunit 2 of NADH dehydrogenase (Complex I of the respiratory chain). This hypothetical gene occurs in the mitochondrial genome of kinetoplastids, a group of unicellular eukaryotes including the causative agents of African sleeping sickness and leishmaniasis. In the present study, we test this assignment by using bioinformatics methods that are highly sensitive in identifying remote homologs and confront the prediction with available biological knowledge.

Results

Comparison of MURF1 profile Hidden Markov Model (HMM) against fu

SUBMITTER: Kannan S 

PROVIDER: S-EPMC2572627 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature