Theory, analysis, and interpretation of single-molecule force spectroscopy experiments.

ABSTRACT: Dynamic force spectroscopy probes the kinetic and thermodynamic properties of single molecules and molecular assemblies. Here, we propose a simple procedure to extract kinetic information from such experiments. The cornerstone of our method is a transformation of the rupture-force histograms obtained at different force-loading rates into the force-dependent lifetimes measurable in constant-force experiments. To interpret the force-dependent lifetimes, we derive a generalization of Bell's formula that is formally exact within the framework of Kramers theory. This result complements the analytical expression for the lifetime that we derived previously for a class of model potentials. We illustrate our procedure by analyzing the nanopore unzipping of DNA hairpins and the unfolding of a protei

SUBMITTER: Dudko OK 

PROVIDER: S-EPMC2572921 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature