Efficient cell-free production of olfactory receptors: detergent optimization, structure, and ligand binding analyses.

ABSTRACT: High-level production of membrane proteins, particularly of G protein-coupled receptors (GPCRs) in heterologous cell systems encounters a number of difficulties from their inherent hydrophobicity in their transmembrane domains, which frequently cause protein aggregation and cytotoxicity and thus reduce the protein yield. Recent advances in cell-free protein synthesis circumvent those problems to produce membrane proteins with a yield sometimes exceeding the cell-based approach. Here, we report cell-free production of a human olfactory receptor 17-4 (hOR17-4) using the wheat germ extract. Using the simple method, we also successful produced two additional olfactory receptors. To obtain soluble olfactory receptors and to increase yield, we directly added different detergents in varying conce

SUBMITTER: Kaiser L 

PROVIDER: S-EPMC2572932 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature