Escherichia coli O157:H7 survives within human macrophages: global gene expression profile and involvement of the Shiga toxins.

ABSTRACT: Escherichia coli O157:H7 is an important food-borne pathogen that specifically binds to the follicle-associated epithelium in the intestine, which rapidly brings this bacterial pathogen in contact with underlying human macrophages. Very little information is available about the interaction between E. coli O157:H7 and human macrophages. We evaluated the uptake and survival of strain EDL933 during infection of human macrophages. Surprisingly, EDL933 survived and multiplied in human macrophages at 24 h postinfection. The global gene expression profile of this pathogen during macrophage infection was determined. Inside human macrophages, upregulation of E. coli O157:H7 genes carried on O islands (such as pagC, the genes for both of the Shiga toxins, and the two iron transport system operons fi

SUBMITTER: Poirier K 

PROVIDER: S-EPMC2573336 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature