Prediction of Sinorhizobium meliloti sRNA genes and experimental detection in strain 2011.

ABSTRACT:

Background

Small non-coding RNAs (sRNAs) have emerged as ubiquitous regulatory elements in bacteria and other life domains. However, few sRNAs have been identified outside several well-studied species of gamma-proteobacteria and thus relatively little is known about the role of RNA-mediated regulation in most other bacterial genera. Here we have conducted a computational prediction of putative sRNA genes in intergenic regions (IgRs) of the symbiotic alpha-proteobacterium S. meliloti 1021 and experimentally confirmed the expression of dozens of these candidate loci in the closely related strain S. meliloti 2011.

Results

Our first sRNA candidate compilation was based mainly on the output of the sRNAPredictHT algorithm. A thorough manual sequence analysis of the curated list re

SUBMITTER: Valverde C 

PROVIDER: S-EPMC2573895 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature