Caenorhabditis elegans mutant allele identification by whole-genome sequencing.

ABSTRACT: Identification of the molecular lesion in Caenorhabditis elegans mutants isolated through forward genetic screens usually involves time-consuming genetic mapping. We used Illumina deep sequencing technology to sequence a complete, mutant C. elegans genome and thus pinpointed a single-nucleotide mutation in the genome that affects a neuronal cell fate decision. This constitutes a proof-of-principle for using whole-genome sequencing to analyze C. elegans mutants.

SUBMITTER: Sarin S 

PROVIDER: S-EPMC2574580 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature