In vitro budding of intralumenal vesicles into late endosomes is regulated by Alix and Tsg101.

ABSTRACT: Endosomes along the degradation pathway leading to lysosomes accumulate membranes in their lumen and thus exhibit a characteristic multivesicular appearance. These lumenal membranes typically incorporate down-regulated EGF receptor destined for degradation, but the mechanisms that control their formation remain poorly characterized. Here, we describe a novel quantitative biochemical assay that reconstitutes the formation of lumenal vesicles within late endosomes in vitro. Vesicle budding into the endosome lumen was time-, temperature-, pH-, and energy-dependent and required cytosolic factors and endosome membrane components. Our light and electron microscopy analysis showed that the compartment supporting the budding process was accessible to endocytosed bulk tracers and EGF receptor. We a

SUBMITTER: Falguieres T 

PROVIDER: S-EPMC2575168 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature