Analysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET.

ABSTRACT:

Background

Genome-wide approaches have begun to reveal the transcriptional networks responsible for pluripotency in embryonic stem (ES) cells. Chromatin Immunoprecipitation (ChIP) followed either by hybridization to a microarray platform (ChIP-chip) or by DNA sequencing (ChIP-PET), has identified binding targets of the ES cell transcription factors OCT4 and NANOG in humans and mice, respectively. These studies have provided an outline of the transcriptional framework involved in maintaining pluripotency. Recent evidence with comparing multiple technologies suggests that expanding these datasets using different platforms would be a useful resource for examining the mechanisms underlying pluripotency regulation.

Results

We have now identified OCT4 and NANOG genomic targets in

SUBMITTER: Mathur D 

PROVIDER: S-EPMC2575516 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature