Characterization of ten novel Ty1/copia-like retrotransposon families of the grapevine genome.

ABSTRACT:

Background

Retrotransposons make a significant contribution to the size, organization and genetic diversity of their host genomes. To characterize retrotransposon families in the grapevine genome (the fourth crop plant genome sequenced) we have combined two approaches: a PCR-based method for the isolation of RnaseH-LTR sequences with a computer-based sequence similarity search in the whole-genome sequence of PN40024.

Results

Supported by a phylogenic analysis, ten novel Ty1/copia families were distinguished in this study. To select a canonical reference element sequence from amongst the various insertions in the genome belonging to each retroelement family, the following screening criteria were adopted to identify the element sequence with: (1) perfect 5 bp-duplication of ta

SUBMITTER: Moisy C 

PROVIDER: S-EPMC2576258 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature