High-speed high-resolution imaging of intercellular immune synapses using optical tweezers.

ABSTRACT: Imaging in any plane other than horizontal in a microscope typically requires a reconstruction from multiple optical slices that significantly decreases the spatial and temporal resolution that can be achieved. This can limit the precision with which molecular events can be detected, for example, at intercellular contacts. This has been a major issue for the imaging of immune synapses between live cells, which has generally required the reconstruction of en face intercellular synapses, yielding spatial resolution significantly above the diffraction limit and updating at only a few frames per minute. Strategies to address this issue have usually involved using artificial activating substrates such as antibody-coated slides or supported planar lipid bilayers, but synapses with these surrogat

SUBMITTER: Oddos S 

PROVIDER: S-EPMC2576363 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature