Multiple subunit fitting into a low-resolution density map of a macromolecular complex using a gaussian mixture model.

ABSTRACT: Recently, electron microscopy measurement of single particles has enabled us to reconstruct a low-resolution 3D density map of large biomolecular complexes. If structures of the complex subunits can be solved by x-ray crystallography at atomic resolution, fitting these models into the 3D density map can generate an atomic resolution model of the entire large complex. The fitting of multiple subunits, however, generally requires large computational costs; therefore, development of an efficient algorithm is required. We developed a fast fitting program, "gmfit", which employs a Gaussian mixture model (GMM) to represent approximated shapes of the 3D density map and the atomic models. A GMM is a distribution function composed by adding together several 3D Gaussian density functions. Because ou

SUBMITTER: Kawabata T 

PROVIDER: S-EPMC2576401 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature