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Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay.


ABSTRACT: This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

SUBMITTER: Guthrie JL 

PROVIDER: S-EPMC2576621 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay.

Guthrie J L JL   Seah C C   Brown S S   Tang P P   Jamieson F F   Drews S J SJ  

Journal of clinical microbiology 20080910 11


This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR. ...[more]

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