Use of Bordetella pertussis BP3385 to establish a cutoff value for an IS481-targeted real-time PCR assay.

ABSTRACT: This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.

SUBMITTER: Guthrie JL 

PROVIDER: S-EPMC2576621 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature