Time-lapse microscopy of Streptomyces coelicolor growth and sporulation.

ABSTRACT: Bacteria from the genus Streptomyces are among the most complex of all prokaryotes; not only do they grow as a complex mycelium, they also differentiate to form aerial hyphae before developing further to form spore chains. This developmental heterogeneity of streptomycete microcolonies makes studying the dynamic processes that contribute to growth and development a challenging procedure. As a result, in order to study the mechanisms that underpin streptomycete growth, we have developed a system for studying hyphal extension, protein trafficking, and sporulation by time-lapse microscopy. Through the use of time-lapse microscopy we have demonstrated that Streptomyces coelicolor germ tubes undergo a temporary arrest in their growth when in close proximity to sibling extension sites. Following

SUBMITTER: Jyothikumar V 

PROVIDER: S-EPMC2576688 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature