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Reconstitution of iron oxidase from sulfur-grown Acidithiobacillus ferrooxidans.

ABSTRACT: The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified.

SUBMITTER: Taha TM 

PROVIDER: S-EPMC2576702 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature

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Reconstitution of iron oxidase from sulfur-grown Acidithiobacillus ferrooxidans.

Taha Taher M TM   Kanao Tadayoshi T   Takeuchi Fumiaki F   Sugio Tsuyoshi T  

Applied and environmental microbiology 20080912 21

The iron oxidation system from sulfur-grown Acidithiobacillus ferrooxidans ATCC 23270 cells was reconstituted in vitro. Purified rusticyanin, cytochrome c, and aa(3)-type cytochrome oxidase were essential for reconstitution. The iron-oxidizing activity of the reconstituted system was 3.3-fold higher than that of the cell extract from which these components were purified. ...[more]

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