Regulatory subunits of PKA define an axis of cellular proliferation/differentiation in ovarian cancer cells.

ABSTRACT:

Background

The regulatory subunit of cAMP-dependent protein kinase (PKA) exists in two isoforms, RI and RII, which distinguish the PKA isozymes, type I (PKA-I) and type II (PKA-II). Evidence obtained from a variety of different experimental approaches has shown that the relative levels of type I and type II PKA in cells can play a major role in determining the balance between cell growth and differentiation. In order to characterize the effect of PKA type I and type II regulatory subunits on gene transcription at a global level, the PKA regulatory subunit genes for RIalpha and RIIbeta were stably transfected into cells of the ovarian cancer cell line (OVCAR8).

Results

RIalpha transfected cells exhibit hyper-proliferative growth and RIIbeta transfected cells revert to a relat

SUBMITTER: Cheadle C 

PROVIDER: S-EPMC2577111 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature