Mechanotransducers in rat pulpal afferents.

ABSTRACT: The hydrodynamic theory suggests that pain associated with stimulation of a sensitive tooth ultimately involves mechanotransduction as a consequence of fluid movement within exposed dentinal tubules. To determine whether putative mechanotransducers could underlie mechanotransduction in pulpal afferents, we used a single-cell PCR approach to screen retrogradely labeled pulpal afferents. The presence of mRNA encoding BNC-1, ASIC3, TRPV4, TRPA1, the alpha, beta, and gamma subunits of ENaC, and the two pore K+ channels (TREK1, TREK2) and TRAAK were screened in pulpal neurons from rats with and without pulpal inflammation. ASIC3, TRPA1, TREK1, and TREK2 were present in approximately 67%, 64%, 14%, and 10% of pulpal neurons, respectively. There was no detectable influence of inflammation on the

SUBMITTER: Hermanstyne TO 

PROVIDER: S-EPMC2577312 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature