Context dependent function of APPb enhancer identified using enhancer trap-containing BACs as transgenes in zebrafish.

ABSTRACT: An enhancer within intron 1 of the amyloid precursor protein gene (APPb) of zebrafish is identified functionally using a novel approach. Bacterial artificial chromosomes (BACs) were retrofitted with enhancer traps, and expressed as transgenes in zebrafish. Expression from both transient assays and stable lines were used for analysis. Although the enhancer was active in specific nonneural cells of the notochord when placed with APPb gene promoter proximal elements its function was restricted to, and absolutely required for, specific expression in neurons when juxtaposed with additional far-upstream promoter elements of the gene. We demonstrate that expression of green fluorescent protein fluorescence resembling the tissue distribution of APPb mRNA requires both the intron 1 enhancer and app

SUBMITTER: Shakes LA 

PROVIDER: S-EPMC2577333 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature