Multiplex sequencing of plant chloroplast genomes using Solexa sequencing-by-synthesis technology.

ABSTRACT: Organellar DNA sequences are widely used in evolutionary and population genetic studies, however, the conservative nature of chloroplast gene and genome evolution often limits phylogenetic resolution and statistical power. To gain maximal access to the historical record contained within chloroplast genomes, we have adapted multiplex sequencing-by-synthesis (MSBS) to simultaneously sequence multiple genomes using the Illumina Genome Analyzer. We PCR-amplified approximately 120 kb plastomes from eight species (seven Pinus, one Picea) in 35 reactions. Pooled products were ligated to modified adapters that included 3 bp indexing tags and samples were multiplexed at four genomes per lane. Tagged microreads were assembled by de novo and reference-guided assembly methods, using previously publish

SUBMITTER: Cronn R 

PROVIDER: S-EPMC2577356 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature