Protein microarrays for highly parallel detection and quantitation of specific proteins and antibodies in complex solutions.

ABSTRACT:

Background

We have developed and tested a method for printing protein microarrays and using these microarrays in a comparative fluorescence assay to measure the abundance of many specific proteins in complex solutions. A robotic device was used to print hundreds of specific antibody or antigen solutions in an array on the surface of derivatized microscope slides. Two complex protein samples, one serving as a standard for comparative quantitation, the other representing an experimental sample in which the protein quantities were to be measured, were labeled by covalent attachment of spectrally resolvable fluorescent dyes.

Results

Specific antibody-antigen interactions localized specific components of the complex mixtures to defined cognate spots in the array, where the relati

SUBMITTER: Haab BB 

PROVIDER: S-EPMC25774 | biostudies-literature | 2001

REPOSITORIES: biostudies-literature