Disease-associated human telomerase RNA variants show loss of function for telomere synthesis without dominant-negative interference.

ABSTRACT: Telomerase adds simple-sequence repeats to chromosome ends to offset the terminal sequence loss inherent in each cycle of genome replication. Inherited mutations in genes encoding subunits of the human telomerase holoenzyme give rise to disease phenotypes including hematopoietic failure and pulmonary fibrosis. Disease-associated variants of the human telomerase RNA are expressed in heterozygous combination with wild-type telomerase RNA. Here, we exploit a sensitized human primary cell assay system to investigate the biological function of disease-linked telomerase RNA variants and their impact on the function of coexpressed wild-type telomerase RNA. We find that telomerase RNA variants discovered in patients with dyskeratosis congenita or aplastic anemia show loss of function without any i

SUBMITTER: Errington TM 

PROVIDER: S-EPMC2577424 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature