Improved coverage of cDNA-AFLP by sequential digestion of immobilized cDNA.

ABSTRACT:

Background

cDNA-AFLP is a transcriptomics technique which does not require prior sequence information and can therefore be used as a gene discovery tool. The method is based on selective amplification of cDNA fragments generated by restriction endonucleases, electrophoretic separation of the products and comparison of the band patterns between treated samples and controls. Unequal distribution of restriction sites used to generate cDNA fragments negatively affects the performance of cDNA-AFLP. Some transcripts are represented by more than one fragment while other escape detection, causing redundancy and reducing the coverage of the analysis, respectively.

Results

With the goal of improving the coverage of cDNA-AFLP without increasing its redundancy, we designed a modified cD

SUBMITTER: Weiberg A 

PROVIDER: S-EPMC2577664 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature