Circulating RNA transcripts identify therapeutic response in cystic fibrosis lung disease.

ABSTRACT:

Rationale

Circulating leukocyte RNA transcripts are systemic markers of inflammation, which have not been studied in cystic fibrosis (CF) lung disease. Although the standard assessment of pulmonary treatment response is FEV(1), a measure of airflow limitation, the lack of systemic markers to reflect changes in lung inflammation critically limits the testing of proposed therapeutics.

Objectives

We sought to prospectively identify and validate peripheral blood leukocyte genes that could mark resolution of pulmonary infection and inflammation using a model by which RNA transcripts could increase the predictive value of spirometry.

Methods

Peripheral blood mononuclear cells were isolated from 10 patients with CF and acute pulmonary exacerbations before and after therapy.

SUBMITTER: Saavedra MT 

PROVIDER: S-EPMC2577728 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature