Influenza A virus inhibits alveolar fluid clearance in BALB/c mice.

ABSTRACT:

Rationale

Pulmonary infections can impair alveolar fluid clearance (AFC), contributing to formation of lung edema. Effects of influenza A virus (IAV) on AFC are unknown.

Objectives

To determine effects of IAV infection on AFC, and to identify intercellular signaling mechanisms underlying influenza-mediated inhibition of AFC.

Methods

BALB/c mice were infected intranasally with influenza A/WSN/33 (10,000 or 2,500 focus-forming units per mouse). AFC was measured in anesthetized, ventilated mice by instilling 5% bovine serum albumin into the dependent lung.

Measurements and main results

Infection with high-dose IAV resulted in a steady decline in arterial oxygen saturation and increased lung water content. AFC was significantly inhibited starting 1 hour after infec

SUBMITTER: Wolk KE 

PROVIDER: S-EPMC2577730 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature