Production of a specific extracellular inhibitor of TRPM3 channels.

ABSTRACT:

Background and purpose

Isoform-specific ion channel blockers are useful for target validation in drug discovery and can provide the basis for new therapeutic agents and aid in determination of physiological functions of ion channels. The aim of this study was to generate a specific blocker of human TRPM3 channels as a tool to help investigations of this member of the TRP cationic channel family.

Experimental approach

A polyclonal antibody (TM3E3) was made to a conserved peptide of the third extracellular (E3) loop of TRPM3 and tested for binding and functional effect. Studies of channel activity were made by whole-cell planar patch-clamp and fura-2 intracellular Ca(2+) measurement.

Key results

Ionic current mediated by TRPM3 was inhibited partially by TM3E3 over a per

SUBMITTER: Naylor J 

PROVIDER: S-EPMC2579657 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature