Preferential gene expression in the limbus of the vervet monkey.

ABSTRACT:

Purpose

To elucidate the unique molecular factors and biological processes that are differentially expressed in the limbal stem cell microenvironment by comparing directly to that of its immediate adjacent structures, the cornea and conjunctiva.

Methods

Total RNA was isolated and amplified from the limbus, cornea, and conjunctiva. A gene expression profile of each tissue type was obtained by using microarray technique. The transcripts in which the expression level was at least twofold higher than that in the other two tissue types were identified. The expression levels of selected genes were confirmed by quantitative reverse transcription polymerase chain reaction (QRT-PCR). Protein expression of selected genes were confirmed by an immunohistochemistry study in normal human

SUBMITTER: Ding Z 

PROVIDER: S-EPMC2579939 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature