Extracellular DNA chelates cations and induces antibiotic resistance in Pseudomonas aeruginosa biofilms.

ABSTRACT: Biofilms are surface-adhered bacterial communities encased in an extracellular matrix composed of DNA, bacterial polysaccharides and proteins, which are up to 1000-fold more antibiotic resistant than planktonic cultures. To date, extracellular DNA has been shown to function as a structural support to maintain Pseudomonas aeruginosa biofilm architecture. Here we show that DNA is a multifaceted component of P. aeruginosa biofilms. At physiologically relevant concentrations, extracellular DNA has antimicrobial activity, causing cell lysis by chelating cations that stabilize lipopolysaccharide (LPS) and the outer membrane (OM). DNA-mediated killing occurred within minutes, as a result of perturbation of both the outer and inner membrane (IM) and the release of cytoplasmic contents, including g

SUBMITTER: Mulcahy H 

PROVIDER: S-EPMC2581603 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature