Crystallization and preliminary X-ray analysis of the NKX2.5 homeodomain in complex with DNA.

ABSTRACT: As part of an effort to elucidate the molecular basis for the pathogenesis of NKX2.5 mutations in congenital heart disease using X-ray crystallography, the NKX2.5 homeodomain has been crystallized in complex with a specific DNA element, the -242 promoter region of atrial natriuretic factor. Crystals of the homeodomain-DNA complex diffracted X-rays to 1.7 A resolution and belonged to space group P6(5), with unit-cell parameters a = b = 71.5, c = 94.3 A. The asymmetric unit contained two molecules of the NKX2.5 homeodomain and one double-stranded oligonucleotide.

SUBMITTER: Genis C 

PROVIDER: S-EPMC2581709 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature