Activation of an IL-6:STAT3-dependent transcriptome in pediatric-onset inflammatory bowel disease.

ABSTRACT:

Background

While activation of the IL-6-dependent transcription factor signal transducer and activator of transcription 3 (STAT3) has been implicated in the pathogenesis of inflammatory bowel disease (IBD), a direct effect on mucosal gene expression and inflammation has not been shown. We hypothesized that a proinflammatory IL-6:STAT3-dependent biological network would be up regulated in pediatric-onset IBD patients, and would be associated with the severity of mucosal inflammation.

Methods

Patients with pediatric-onset IBD were enrolled at diagnosis and during therapy. Serum cytokine analysis was performed using Bioplex. STAT3 phosphorylation (pSTAT3) in peripheral blood leukocytes (PBLs) was assessed by flow cytometry. Immunohistochemistry of colonic mucosa was used to loc

SUBMITTER: Carey R 

PROVIDER: S-EPMC2581837 | biostudies-literature | 2008 Apr

REPOSITORIES: biostudies-literature