A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors.

ABSTRACT: Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the PhiC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis.

SUBMITTER: Bateman JR 

PROVIDER: S-EPMC2581973 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature