Arginyltransferase regulates alpha cardiac actin function, myofibril formation and contractility during heart development.

ABSTRACT: Post-translational arginylation mediated by arginyltransferase (Ate1) is essential for cardiovascular development and angiogenesis in mammals and directly affects myocardium structure in the developing heart. We recently showed that arginylation exerts a number of intracellular effects by modifying proteins involved in the functioning of the actin cytoskeleton and in cell motility. Here, we investigated the role of arginylation in the development and function of cardiac myocytes and their actin-containing structures during embryogenesis. Biochemical and mass spectrometry analyses showed that alpha cardiac actin undergoes arginylation at four sites during development. Ultrastructural analysis of the myofibrils in wild-type and Ate1 knockout mouse hearts showed that the absence of arginylati

SUBMITTER: Rai R 

PROVIDER: S-EPMC2582055 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature