Enhancement of protein sensitivity for MALDI imaging mass spectrometry after chemical treatment of tissue sections.

ABSTRACT: MALDI imaging mass spectrometry (IMS) has become a valuable tool for the investigation of the content and distribution of molecular species in tissue specimens. Numerous methodological improvements have been made to optimize tissue section preparation and matrix deposition protocols, as well as MS data acquisition and processing. In particular for proteomic analyses, washing the tissue sections before matrix deposition has proven useful to improve spectral qualities by increasing ion yields and the number of signals observed. We systematically explore here the effects of several solvent combinations for washing tissue sections. To minimize experimental variability, all of the measurements were performed on serial sections cut from a single mouse liver tissue block. Several other key steps

SUBMITTER: Seeley EH 

PROVIDER: S-EPMC2582528 | biostudies-literature | 2008 Aug

REPOSITORIES: biostudies-literature