Endoplasmic reticulum stress markers are associated with obesity in nondiabetic subjects.

ABSTRACT:

Objective

Adipocyte and hepatocyte endoplasmic reticulum (ER) stress response is activated in dietary and genetic models of obesity in mice. We hypothesized that ER stress was also activated and associated with reduced insulin sensitivity (SI) in human obesity.

Research design and methods

We recruited 78 healthy, nondiabetic individuals over a spectrum of body mass index (BMI) who underwent oral and iv glucose tolerance tests, and fasting sc adipose and muscle biopsies. We tested expression of 18 genes and levels of total and phosphorylated eukaryotic initiation factor 2alpha, c-jun, and c-Jun N-terminal kinase 1 in adipose tissue. We compared gene expression in stromal vascular and adipocyte fractions in paired samples from 22 individuals, and tested clustering on gene and

SUBMITTER: Sharma NK 

PROVIDER: S-EPMC2582561 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature