Runx expression is mitogenic and mutually linked to Wnt activity in blastula-stage sea urchin embryos.

ABSTRACT:

Background

The Runt homology domain (Runx) defines a metazoan family of sequence-specific transcriptional regulatory proteins that are critical for animal development and causally associated with a variety of mammalian cancers. The sea urchin Runx gene SpRunt-1 is expressed throughout the blastula stage embryo, and is required globally during embryogenesis for cell survival and differentiation.

Methodology/principal findings

Depletion of SpRunt-1 by morpholino antisense-mediated knockdown causes a blastula stage deficit in cell proliferation, as shown by bromodeoxyuridine (BrdU) incorporation and direct cell counts. Reverse transcription coupled polymerase chain reaction (RT-PCR) studies show that the cell proliferation deficit is presaged by a deficit in the expression of s

SUBMITTER: Robertson AJ 

PROVIDER: S-EPMC2582955 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature