Introduction of the chloroplast redox regulatory region in the yeast ATP synthase impairs cytochrome c oxidase.

ABSTRACT: The ATP synthase is under a number of mechanisms of regulation. The chloroplast ATPase has a unique mode of regulation in which activity is controlled by the redox state in the organelle. This mode of regulation is determined by a small unique region within the gamma-subunit and this region contains two cysteine residues. Introduction of this region within the yeast gamma-subunit causes a defect in oxidative phosphorylation. Oxidative phosphorylation is restored if the cysteine residues are replaced with serine. Biochemical analysis of the chimeric mitochondrial ATPase indicates that the ATP synthase is not largely altered with the cysteine residues in either the oxidized or reduced states. However, the level and activity of cytochrome c oxidase are decreased by about 90%, whereas that of

SUBMITTER: Shen H 

PROVIDER: S-EPMC2583308 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature