Global transcription and metabolic flux analysis of Escherichia coli in glucose-limited fed-batch cultivations.

ABSTRACT: A time series of whole-genome transcription profiling of Escherichia coli K-12 W3110 was performed during a carbon-limited fed-batch process. The application of a constant feed rate led to the identification of a dynamic sequence of diverse carbon limitation responses (e.g., the hunger response) and at the same time provided a global view of how cellular and extracellular resources are used: the synthesis of high-affinity transporters guarantees maximal glucose influx, thereby preserving the phosphoenolpyruvate pool, and energy-dependent chemotaxis is reduced in order to provide a more economic "work mode." sigma(S)-mediated stress and starvation responses were both found to be of only minor relevance. Thus, the experimental setup provided access to the hunger response and enabled the diff

SUBMITTER: Lemuth K 

PROVIDER: S-EPMC2583496 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature