Optimisation of region-specific reference gene selection and relative gene expression analysis methods for pre-clinical trials of Huntington's disease.

ABSTRACT:

Background

Transcriptional dysregulation is an early, key pathogenic mechanism in Huntington's disease (HD). Therefore, gene expression analyses have biomarker potential for measuring therapeutic efficacy in pre-clinical trials, particularly those aimed at correcting gene expression abnormalities. Housekeeping genes are commonly used as endogenous references in gene expression studies. However, a systematic study comparing the suitability of candidate reference genes for use in HD mouse models has not been performed. To remedy this situation, 12 housekeeping genes were examined to identify suitable reference genes for use in expression assays.

Results

We found that commonly used reference genes are dysregulated at later time points in the R6/2 mouse model of HD. Therefore, i

SUBMITTER: Benn CL 

PROVIDER: S-EPMC2584034 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature