Differential response of human basophil activation markers: a multi-parameter flow cytometry approach.

ABSTRACT:

Background

Basophils are circulating cells involved in hypersensitivity reactions and allergy but many aspects of their activation, including the sensitivity to external triggering factors and the molecular aspects of cell responses, are still to be focused. In this context, polychromatic flow cytometry (PFC) is a proper tool to investigate basophil function, as it allows to distinguish the expression of several membrane markers upon activation in multiple experimental conditions.

Methods

Cell suspensions were prepared from leukocyte buffy coat of K2-EDTA anticoagulated blood specimens; about 1500-2500 cellular events for each tested sample, gated in the lymphocyte CD45dim area and then electronically purified as HLADRnon expressing/CD123bright, were identified as basophilic

SUBMITTER: Chirumbolo S 

PROVIDER: S-EPMC2584049 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature