Determining the impact of alternative splicing events on transcriptome dynamics.

ABSTRACT:

Background

The complete sequencing of the human genome and its subsequent analysis revealed a predominant role for alternative splicing in the generation of proteome diversity. Splice switching oligonucleotides (SSOs) are a powerful and specific tool to experimentally control alternative splicing of endogenous messenger RNAs in living cells. SSOs also have therapeutic potential to treat diseases that are caused by aberrant splicing. The assignment of biological roles to alternative splicing events of currently unknown function promises to provide a largely untapped source of potential new therapeutic targets. Here we have developed a protocol that combines high sensitivity microarrays with the transfection of SSOs to monitor global changes in gene expression downstream of alternate

SUBMITTER: Wilhelm E 

PROVIDER: S-EPMC2584107 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature