Integrating chromosomal aberrations and gene expression profiles to dissect rectal tumorigenesis.

ABSTRACT:

Background

Accurate staging of rectal tumors is essential for making the correct treatment choice. In a previous study, we found that loss of 17p, 18q and gain of 8q, 13q and 20q could distinguish adenoma from carcinoma tissue and that gain of 1q was related to lymph node metastasis. In order to find markers for tumor staging, we searched for candidate genes on these specific chromosomes.

Methods

We performed gene expression microarray analysis on 79 rectal tumors and integrated these data with genomic data from the same sample series. We performed supervised analysis to find candidate genes on affected chromosomes and validated the results with qRT-PCR and immunohistochemistry.

Results

Integration of gene expression and chromosomal instability data revealed similarit

SUBMITTER: Lips EH 

PROVIDER: S-EPMC2584339 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature