A general SNP-based molecular barcode for Plasmodium falciparum identification and tracking.

ABSTRACT:

Background

Single nucleotide polymorphism (SNP) genotyping provides the means to develop a practical, rapid, inexpensive assay that will uniquely identify any Plasmodium falciparum parasite using a small amount of DNA. Such an assay could be used to distinguish recrudescence from re-infection in drug trials, to monitor the frequency and distribution of specific parasites in a patient population undergoing drug treatment or vaccine challenge, or for tracking samples and determining purity of isolates in the laboratory during culture adaptation and sub-cloning, as well as routine passage.

Methods

A panel of twenty-four SNP markers has been identified that exhibit a high minor allele frequency (average MAF > 35%), for which robust TaqMan genotyping assays were constructed. All

SUBMITTER: Daniels R 

PROVIDER: S-EPMC2584654 | biostudies-literature | 2008 Oct

REPOSITORIES: biostudies-literature