Isolation, characterization and comparison of Atlantic and Chinook salmon growth hormone 1 and 2.

ABSTRACT:

Background

Growth hormone (GH) is an important regulator of skeletal growth, as well as other adapted processes in salmonids. The GH gene (gh) in salmonids is represented by duplicated, non-allelic isoforms designated as gh1 and gh2. We have isolated and characterized gh-containing bacterial artificial chromosomes (BACs) of both Atlantic and Chinook salmon (Salmo salar and Oncorhynchus tshawytscha) in order to further elucidate our understanding of the conservation and regulation of these loci.

Results

BACs containing gh1 and gh2 from both Atlantic and Chinook salmon were assembled, annotated, and compared to each other in their coding, intronic, regulatory, and flanking regions. These BACs also contain the genes for skeletal muscle sodium channel oriented in the same direct

SUBMITTER: von Schalburg KR 

PROVIDER: S-EPMC2584663 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature