PCNA interacts with Prox1 and represses its transcriptional activity.

ABSTRACT:

Purpose

Prox1 is a transcription factor which can function either as a transcriptional activator, transcriptional repressor or a transcriptional corepressor. This paper seeks to better understand the role of protein-protein interactions in this multitude of functions.

Methods

We performed a yeast two-hybrid screen of an 11.5 day post coitum (dpc) mouse embryo cDNA library using the homeo-Prospero domain of Prox1 as bait. Computer modeling, cotransfection analysis and confocal immunolocalization were used to investigate the significance of one of the identified interactions.

Results

Proliferating cell nuclear antigen (PCNA) was identified as a Prox1 interacting protein. Prox1 interactions with PCNA require the PCNA interacting protein motif (PIP box), located in the Pr

SUBMITTER: Chen X 

PROVIDER: S-EPMC2584773 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature