Differential gene expression associated with postnatal equine articular cartilage maturation.

ABSTRACT:

Background

Articular cartilage undergoes an important maturation process from neonate to adult that is reflected by alterations in matrix protein organization and increased heterogeneity of chondrocyte morphology. In the horse, these changes are influenced by exercise during the first five months of postnatal life. Transcriptional profiling was used to evaluate changes in articular chondrocyte gene expression during postnatal growth and development.

Methods

Total RNA was isolated from the articular cartilage of neonatal (0-10 days) and adult (4-5 years) horses, subjected to one round of linear RNA amplification, and then applied to a 9,367-element equine-specific cDNA microarray. Comparisons were made with a dye-swap experimental design. Microarray results for selected genes

SUBMITTER: Mienaltowski MJ 

PROVIDER: S-EPMC2585085 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature