The role of interleukin-12 in the heavy metal-elicited immunomodulation: relevance of various evaluation methods.

ABSTRACT:

Background

Increasing evidence exists that heavy metals modulate T helper cell (Th) responses and thereby elicit various pathological manifestation. Interleukin (IL)-12, a crucial innate cytokine, was found to be regulated by such xenobiotic agents. This study aimed at testing whether IL-12 profiles may be indicative of heavy metals-induced immunomodulation.

Methods

Human immunocompetent cells, activated either by monoclonal antibodies or heat-killed Salmonella enterica, were cultured in the absence or presence of cadmium (Cd) acetate or mercuric (Hg) chloride. In vivo experiments were set up where BALB/c mice were exposed to sub-lethal doses of Cd or Hg salts for 3 or 5 weeks. Cytotoxicity was assessed by MTT-reduction assay. Modulation of cytokine profiles was evaluated by

SUBMITTER: Hemdan NY 

PROVIDER: S-EPMC2585571 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature