Systems analysis unfolds the relationship between the phosphoketolase pathway and growth in Aspergillus nidulans.

ABSTRACT:

Background

Aspergillus nidulans is an important model organism for studies on fundamental eukaryotic cell biology and on industrial processes due to its close relation to A. niger and A. oryzae. Here we identified the gene coding for a novel metabolic pathway in A. nidulans, namely the phosphoketolase pathway, and investigated the role of an increased phosphoketolase activity.

Methodology/principal findings

Over-expression of the phosphoketolase gene (phk) improved the specific growth rate on xylose, glycerol and ethanol. Transcriptome analysis showed that a total of 1,222 genes were significantly affected by over-expression of the phk, while more than half of the affected genes were carbon source specific. During growth on glucose medium, the transcriptome analysis showed t

SUBMITTER: Panagiotou G 

PROVIDER: S-EPMC2585806 | biostudies-literature | 2008

REPOSITORIES: biostudies-literature