Characterization of protein dynamics in asymmetric cell division by scanning fluorescence correlation spectroscopy.

ABSTRACT: The development and differentiation of complex organisms from the single fertilized egg is regulated by a variety of processes that all rely on the distribution and interaction of proteins. Despite the tight regulation of these processes with respect to temporal and spatial protein localization, exact quantification of the underlying parameters, such as concentrations and distribution coefficients, has so far been problematic. Recent experiments suggest that fluorescence correlation spectroscopy on a single molecule level in living cells has great promise in revealing these parameters with high precision. The optically challenging situation in multicellular systems such as embryos can be ameliorated by two-photon excitation, where scattering background and cumulative photobleaching is limi

SUBMITTER: Petrasek Z 

PROVIDER: S-EPMC2586573 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature