System-level mapping of Escherichia coli response regulator dimerization with FRET hybrids.

ABSTRACT:

Summary

Two-component signal transduction, featuring highly conserved histidine kinases (HKs) and response regulators (RRs), is one of the most prevalent signalling schemes in prokaryotes. RRs function as phosphorylation-activated switches to mediate diverse output responses, mostly via transcription regulation. As bacterial genomes typically encode multiple two-component proteins for distinct signalling pathways, the sequence and structural similarities of RR receiver domains create significant challenges to maintain interaction specificity. It is especially demanding for members of the OmpR/PhoB subfamily, the largest RR subfamily, which share a conserved dimerization interface for phosphorylation-mediated transcription regulation. We developed a strategy to investigate RR intera

SUBMITTER: Gao R 

PROVIDER: S-EPMC2586830 | biostudies-literature | 2008 Sep

REPOSITORIES: biostudies-literature