Hydrocortisone modulates cholera toxin endocytosis by regulating immature enterocyte plasma membrane phospholipids.

ABSTRACT:

Background & aims

Diarrheal disease is a major cause of morbidity and mortality in infants and children worldwide. Evidence has indicated immature human enterocytes and their interaction with bacteria and enterotoxins may account for the noted increased susceptibility of neonates to diarrhea. Our aim was to characterize the developmental difference in cholera toxin (CT)-GM1-mediated endocytosis.

Methods

We used H4 cells (a fetal human small intestinal epithelial cell line), T84 cells, primary cultured mature human small intestinal epithelial cells, and human fetal small intestine xenografts. In addition, hydrocortisone was used as a potent intestinal trophic factor to induce maturation of the human enterocytes.

Results

Here we show an increase in CT-caveolae and a dec

SUBMITTER: Lu L 

PROVIDER: S-EPMC2587123 | biostudies-literature | 2008 Jul

REPOSITORIES: biostudies-literature