Ontology highlight
ABSTRACT:
SUBMITTER: Wahlestedt C
PROVIDER: S-EPMC25880 | biostudies-literature | 2000 May
REPOSITORIES: biostudies-literature
Wahlestedt C C Salmi P P Good L L Kela J J Johnsson T T Hökfelt T T Broberger C C Porreca F F Lai J J Ren K K Ossipov M M Koshkin A A Jakobsen N N Skouv J J Oerum H H Jacobsen M H MH Wengel J J
Proceedings of the National Academy of Sciences of the United States of America 20000501 10
Insufficient efficacy and/or specificity of antisense oligonucleotides limit their in vivo usefulness. We demonstrate here that a high-affinity DNA analog, locked nucleic acid (LNA), confers several desired properties to antisense agents. Unlike DNA, LNA/DNA copolymers were not degraded readily in blood serum and cell extracts. However, like DNA, the LNA/DNA copolymers were capable of activating RNase H, an important antisense mechanism of action. In contrast to phosphorothioate-containing oligo ...[more]